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文献和实验: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
Models of Polycystic Kidney Disease
, and aortic and cerebral aneurysms (1 ). ARPKD is encountered less frequently. PKHD1 , a locus on human Chr 6p21-cen that predisposes individuals to develop ARPKD, has been reported. ARPKD patients primarily develop cysts in the collecting ducts, with hepatic
and congenital hepatic fibrosis) and in PCK rats are speculated to be related to mutations to polycystic kidney and hepatic disease 1 (PKHD1 ) which have been recently demonstrated, though the exact causal relation between these mutations and hepatobiliary
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