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文献和实验) is accompanied with or inactivates the GSK activity. However, it is still not conclusive what the role of Tyr phosphorylation (Y216 fro beta, Y279 for alpha) on GSK activity is. One reference is a review paper published in Cell in 2001, you may take a quick
Making antibodies to synthetic peptides
albumin, or keyhole limpet hemocyanin (our favorite, from Calbiochem #374817). Good coupling methods are glutaraldehyde (amino to amino), MBS (=m-Maleimidobenzoic acid-N-hydroxysuccinimide; amino to sulfhydryl), BDB (bisdiazobenzidine; couples tyr
Making antibodies to synthetic pepti
; couples tyr to tyr), EDAC (carbodiimide; couples amino to carboxyl). We often add an extra ("adventitious") tyr to the N- or C-terminus of the synthetic peptide, for BDB coupling. We've done this more often than not. Try to put the extra tyr
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