- ￥4373
- 赛默飞（Thermo Fisher）
- MA527885
- 2025年07月14日
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文献和实验on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications. To distinguish between alleles at loci of interest in precipitated chromatin fractions, we use "hot-stop" PCR (Steps 1-22) or SSCP (Steps 23-37). See Uejima et al. (2000
95 19:59:00 GMT Organization: CuraGen Corporation Message-ID: References: 446bk4$1sm@mserv1.dl.ac.uk> Methylene Blue Staining Run the oligo into a mini PAGE and when finished, soak the gel in a soln of 0.0002% Methylene Blue and 0.1X TBE
in H2O. Soak membrane in transfer buffer for 10 min. 3. Set up transfer from the gel to a nylon membrane in transfer buffer. 4. Place “transfer sandwich” in semi-dry transfer chamber. Run at 23 V for 30 min for 0.75 and 1.0 mm gels or 40 min
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