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文献和实验times and put on ice for 15 min 5、 Spin 14000g for 15 min Room Temperature and clear supernatant (700 μl) transfer to miniprep column 6、 Spin at 14000 g for 1 min, washed with 700 μl wash buffer once 7、 Spin empty column to discard the rest
Lipoprotein Analysis Week 2: Electrophoresis
buffer has a low pH (6.8) and contains Cl-. At the low pH of the stacking gel, the Cl- in the stacking gel are negatively charged and hence move towards the anode (+), but the glycine entering from the gel buffer has only a very small negative charge (pI
Maxiprep of plasmid DNA from E. coli
Ingredients Ingredients are per culture; make enough for one extra culture to allow for pipetting error). 150μL sterile 50% glycerol 1mL TEG (25mM Tris-Cl, 10mM EDTA, 50mM dextrose) 111μL 20mg/mL lysozyme 2mL
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