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文献和实验Clonality - X Chromosome Inactivation Assay
can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
我也准备做关于wnt通路的,我做侵袭方面的。楼主可否多多交流。向你学习?QQ674693586.忘多指教 ylhuang0502 向楼主强烈推荐Cell上的一篇Review: Wnt pathway activation: new relations and locations, Cell. 2005 Jan 14;120(1):11-4. The full size paper can be available
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