- ￥4316
- 赛默飞（Thermo Fisher）
- MA524156
- 2025年07月16日
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文献和实验Bromodeoxyuridine Staining of Mammary Tissue
from a post-pubertal mouse.) II. BrdU Detection A. Perfusion fixed, paraffin sections: 1. Deparaffinize sections using standard xylene/alcohol procedures. 2. Treat sections with 0.1% Trypsin in 0.1M TBS (pH7.5) + 0.1% CaCl2 for 20 minutes at 37
. Avoid repeated freeze-thaw cycles. All enzymes, upon reconstitution, can be sterile filtered through a 0.22 µm pore size membrane. Generally most of the enzymes used in cell isolation procedures (except trypsin) can be directly
, or algae may be used.) H-thymidine with at least 4 µc/ml, 0.36 c/mM Phosphate buffered saline (PBS), Trypsin Methanol:Acetic acid (3:1) fixative Nuclear track emulsion and equipment for autoradiographic analysis Subbed slides, coverslips
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