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文献和实验LABELING ALPHA-ACTININ WITH IATR
with good stirring.The volume (ml)of borate buffer = mg IATR x 9 / (mg/ml)alpha-actinin. 4.Clarify IATR solution at 25,000 rpm,4℃,for 10 min in a 42.2Ti rotor. 5.Mix equal volumes of clarified dye and alpha-actinin in a test tube; remove
ALPHA FACTOR ARREST AND RELEASE
of approximately 3 x 10�5 M, while bar1 strains require much lower pheromone concentrations (10-8 M). To release cells from alpha factor arrest, spin down cells and wash twice (using a large volume, dependent on the size of the cell pellet anticipated
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
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