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文献和实验HSV Latency In Vitro: In Situ Hybridization Methods
(4 ). Latency is maintained in neurons in culture for as long as 10 wk in the presence of NGF. Viral transcripts and antigens associated with the productive infection are not detected during the latent infection (2 ,3 ,5 ). Viral transcription is restricted
Measurements of Active TGF Generated by Culture Cells
-β1, 2, and 3 (3 –5 ). Most cell lines and tissues secrete TGF-β as a large latent complex formed by three components: TGF-β, LAP (latency-associated protein), and LTBP (latent TGF-β binding proteins). TGF-β is noncovalently associated to its prodomain LAP (6 –
Delivery Using Herpes Simplex Virus: An Overview
the ability to establish persistent quiescent genomes in neurons, but that are unable to subsequently reactivate in the nervous system. These persistent genomes are devoid of lytic gene expression, but retain the ability to express latency-associated
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