- ￥5134
- 赛默飞（Thermo Fisher）
- MA521265
- 2025年07月12日
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文献和实验Calpain Methods in Hepatic Ischemia-Reperfusion Injury
activate several calcium-dependent systems including calpain-like proteases (8 –11 ). Calpain-like proteolytic activity has recently been found to be involved in the pathogenesis of the ischemic brain (12 ), heart (13 ), kidney (14
Rat Renal Proximal Tubules, Hypoxia, lonomycin, and Calpain
by collagenase digestion and Percoll centrifugation has recently been used by us for the study of calpain activity in this tissue (11 –13 ). Briefly, the kidneys of two male Sprague-Dawley rats are perfused, and the cortices are digested
with TBS-T 3 times for 10 min each.11. Make dilution of secondary antibody (usually 1 μl in 10 ml), and cover membrane with antibody solution and shake on a platform for 1 h at room temp.12. Discard antibody solution, and wash membrane with TBS-T 4 times
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