- ￥5921
- 赛默飞（Thermo Fisher）
- MA517975
- 2025年07月15日
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N CONJUGATED (PK136)
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文献和实验Assessing Licensing of NK Cells
, splenocytes are stimulated with plate-bound anti-NK1.1 monoclonal antibody in the presence of brefeldin A and then assessed for NK cell activation on a single-cell basis using intracellular cytokine interferon-γ staining and flow cytometry.
media (= RPMI 1640 + 10% FBS) PBS + 2% EDTA Triton X-100 solution (10% in dH2 O) Effector cell preparation: The mouse NK-cell line KY1 is used as an effector cell. During recovery (e.g. after freezing), these cells require 1000 U
Hybrid Resistance by Mouse NK Cells In Vitro
, leads to generation of lytic NK1.1+ Ly49- cells whose function is similar to that of fetal NK cells, i.e., they can lyse YAC-1 tumor cells and class I deficient cells, but cannot lyse normal lymphoblasts and lack Ly49 receptors (5 ). These two types
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