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文献和实验Quantitative PCR for cAMP RI Alpha mRNA: Use of Site-Directed Mutation and PCR Mimics
assays, Serial Analysis of Gene Expression (SAGE), and many other techniques; however, for single transcripts, especially where tissue is limited or abundance is low, quantitative polymerase chain reaction (PCR) is the method of choice
after specifically interacting with its receptors. Human IL-2 is a 133-amino acid polypeptide with a molecular mass of 15-18 kDa. IL-2 signaling is mediated by a multichain receptor complex consisting of an alpha (CD25), beta (CD122), and gamma (CD132) chain. The IL-2R
motifs located prevalently in the 3′ untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based
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