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文献和实验Flow Cytometric Analysis Of Bcl Family members
against Bcl-xL (Santacruz Biotechnology, USA) and rabbit polyclonal against Bax (Santacruz Biotechnology, USA). The secondary AB against Bcl2 was goat anti mouse Ig FITC (Immunotech, USA) and against Bcl-xL and Bax goat anti rabbit FITC (ICN-Cappel, USA). FITC
Double immunofluorescence staining for BCL-6 and else
antibodies: rabbit anti-BCL-6 0.5µg/ml in PBS/BSA/Azide + mouse anti HA supn 1:100. Secondary antibodies: biotin-conjugated goat anti rabbit (1:200) + TRITC conjugated goat anti-mouse (1:200). Alternatively, biotin-conjugated goat anti mouse (1:200
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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