- ￥5222
- 赛默飞（Thermo Fisher）
- MA516546
- 2025年07月13日
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文献和实验Th17 Polarization of Mouse CD4 Cells
实验试剂 Sterile PBS Cell culture medium Sterile plastic petridishes RBC Lysis Buffer [Details: Biolengend, cat.#420301] Anti-mouse CD3ε, clone145-2C11[Details: LEAF™format, cat.#100314]
, Buckinghamshire, UK). 2.3. Immunoblotting 1. Transfer buffer (10×): 250 mM Tris-Base and 1.92 M glycine. Store at RT. 2. Polyvinylidene difluoride (PVDF) membrane from Millipore(Bedford, MA) and gel blot paper from ISCBioExpress(Kaysville and UT
) or VE-8 (F) and the VP64 activator domain, stained with anti-CD144 and analyzed by FACS. Red, cells infected with the pMX vector containing the same DNA-binding domain but linked to the KRAB repression domain (SKD), and stained with anti-CD144 (anti-VE
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