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文献和实验using Dynabeads® CD3⁄CD28 CTS™. Following separation, the CD3 T cells are cultured in the presence of the beads. By combining anti-CD3 and anti-CD28 antibodies on Dynabeads®, the beads will provide both the primary and co-stimulatory signals
) or VE-8 (F) and the VP64 activator domain, stained with anti-CD144 and analyzed by FACS. Red, cells infected with the pMX vector containing the same DNA-binding domain but linked to the KRAB repression domain (SKD), and stained with anti-CD144 (anti-VE
、结果1、MTT法测定结果为了检验嵌合抗CD20Fab’片段对Raji细胞,的生长抑制作用,以PBS为阴性对照,用MTT法测定Fab’片段对Raji细胞生长的影响,其结果显示,Fab’片段对Raji细胞具有抑制作用,其抑制作用具有明显的剂量依赖性,其半数抑制量为(24.2±7.8) µg/ml。表1嵌合抗体CD20 Fab’片段对Raji细胞的生长抑制率Tab 1 The inhibition rate of Raji cells by the anti-CD20 chimeric fragment Fab
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