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文献和实验Breeding and Genotyping of Tfam Conditional Knockout Mice
Respiratory chain dysfunction is an important contributor to human pathology (1 –3 ). The generation of animal models has much facilitated in-depth studies of pathogenetic mechanisms in mitochondrial disease (4 ). The function
Methods for Assessing Binding of Mitochondrial Transcription Factor A (TFAM) to DNA
It is now recognized that mammalian mitochondrial DNA forms a higher structure called the nucleoid, corresponding to the nucleosome of nuclear DNA. Mitochondrial transcription factor A (TFAM), which was cloned as a transcription factor
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
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