- ￥5053
- 赛默飞（Thermo Fisher）
- MA515909
- 2025年07月15日
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文献和实验antibody dilution buffer: TBS-T supplemented with2% (w/v) BSA. 6. Antibodies: Phospho-b-catenin Ser33/Ser37/Thr41, phospho-glycogen synthase Ser641, and glycogen synthase (CellSignaling Technologies, Beverly, MA); b-catenin and GSK-3b(BD PharMingen, San
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
antibodies wereused; GSK3α/b (pS21/9) (#9331; Cell Signaling Technology, Danvers, MA), GSK3a/b (pY279/216) (#612312; BDTransduction Laboratories, San Jose, CA) and GSK3a/b (#05-903; Upstate; Millipore, Billerica, MA) (see Note
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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