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文献和实验-terminal 12 kDa of 14-3-3. Activation-modulated association of 14-3-3 proteins with Cbl in T cellsLiu YC; Elly C; Yoshida H; Bonnefoy-Berard N; Altman A Division of Immunobiology, La Jolla Institute for Allergy and Immunology, La Jolla, California
Introduction of human 14-3-3 proteins
----------------------------------------------------------------------------Activation-modulated association of 14-3-3 proteins with Cbl in T cellsLiu YC; Elly C; Yoshida H; Bonnefoy-Berard N; Altman ADivision of Immunobiology,La Jolla Institute for Allergy and Immunology,La Jolla,California 92037
). 2. Stacking buffer (4×): 0.5 M Tris-HCl, pH 6.8, and 0.4%(w/v) SDS. Store at RT. 3. 30% acrylamide/bis solution (37.5:1 with 2.6% C) andN , N , N , N ￠-tetramethyl-ethylenediamine (TEMED; Bio-Rad,Hercules, CA). 4. Ammonium persulfate: prepare 10% (w/v
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