- ￥4814
- 赛默飞（Thermo Fisher）
- MA515817
- 2025年07月09日
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文献和实验—— co-IP 检测 Hsp90 与 Cdc37 结合情况1、实验材料SKBR3 细胞，PMSF（Amresco，cat:329-98-6），cocktail（上海晶莱生物），脱脂奶粉（cat:66196131T），β-actin（上海晶莱生物）。Anti-Hsp90(santa,cat:sc-69703)、Anti-Cdc37(santa,cat:sc-13129)、Protein A+G agrose(Beyotime)，细胞刮，4 度离心机（eppendorf，centrifuge
Generation of Antibodies to Cell Surface Markers on Mature Natural Killer Cells
an (immunoreceptor tyrosine-based inhibiting motif (ITIM) in the cytoplasmic domain (1 ). In the human system, the NKG2 family of receptor molecules with or without ITIMs associate as heterodimers with CD94 molecules (2 ), and are therefore candidates for murine
Intracellular Cytokine Staining Protocol
are first activated in vitro, stained for surface antigens, as in the regular staining protocol, then fixed and permeabilized to allow for anti-cytokine antibodies to stain intracellularly. In vitro stimulation of cells is usually required for detection of cytokines
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