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文献和实验.Therefore,if you use beta-actin as internal control in Western Blot,you have to think about these circumistance,as though beta-actin is the most popular protein sign to be as internal control.
-time PCR时,本人不懂操作,将β-actin内参设置成target了,现在跑完了,有扩增曲线图,和融解曲线,但没法导出Ct值(SDS 1.4版本的软件), 请教大虾们有没有补救办法,还是只能重新跑了? 黑色的就是我的beta-actin内参 A2: 重新设置一下不行吗,荧光信号已经收集了。不用重跑。 Q3: 最近在做RT-PCR,但始终摸不清β- actin的意义。哪位可以详细介绍一下β- actin作为内参照的意义?它与目的核酸片段的对比能说明什么问题?谢谢
Which housekeeping genes for qRT-PCR control-Real-Time
for you -mema- hi i use beta actin as as positive control for my qRT PCR . i think this is one of the most popular positive control -SHIVA KESHAVA- I dont think there is a real answer to this question. I would use a panel of genes
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