- ￥4253
- 赛默飞（Thermo Fisher）
- MA515623
- 2025年07月11日
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文献和实验: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
Brain Synaptosomal Preparation
at 1000 g at RT. Remove 100 ul for synaptophysin analysis. 3. Layer supernatant onto sucrose and centrifuge again. 4. Carefully layer the supernatant onto 4 ml of 1.2 M sucrose in a SW41 centrifuge tube (Beckman). 5. Spin at 160,000 g
Immunofluorescence or IF is a technique allowing the visualization of a specific protein or antigen in cells or tissues by binding a specific antibody chemically conjugated with a fluorescence dye. Immunofluorescent staining is widely used
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