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文献和实验上皮及间皮类常用标记抗体EPITHELIAL AND MESOTHELIAL MARKERSCK (MNF 116)- MNF-116 is a pan-cytokeratin antibody.- Source: Dakopatts.- Dilution 1:50.- Antigen retrieval method: HIER (pressure cooking) using EDTA.- Staining pattern: Cytoplasmic, typically
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
of First Hospital of Harbin Medical University. RPMI 1640 was purchased from GIBCO. Propidium iodide and Rnase were from Sigma Chemical Co. A murine monoclonal antibody against human Bcl-2 and Bax oncoprotein and antimi ce rabbit polyclonal antibody were purchased
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