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文献和实验| PubMed | ChemPort | 8. Burnett, J. R. & Barrett, P. H. Apolipoprotein B metabolism: tracer kinetics, models, and metabolic studies. Crit. Rev. Clin. Lab. Sci. 39, 89–137 (2002) | PubMed | ChemPort | 9. Farese, R. V. Jr, Ruland, S. L., Flynn, L. M
Buffers:TBS: 25 ml of 1 M Tris-7.5, 30 ml of 5 M NaCl, bring volume up to 1000 ml with ddwaterTBS-T: TBS + 0.5 ml of Tween 205X SDS-PAGE running buffer: 15.1 g Tris base, 94 g glycine in 900 ml water, then add 50 ml of 10% solution of SDS
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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