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文献和实验v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
markers(Bio-Rad). 5. Coomassie Brilliant Blue (CBB) staining solution: 0.025 %(w/v) CBB (Pierce), 40 % (v/v) methanol, and 7 % (v/v)acetic acid. Dissolve CBB in methanol by stirring, followed byfiltration. Then add acetic acid and bring to final
Studies of the Ubiquitin Proteasome System
from other investigators) Transfection efficiency control plasmid, e.g., GFP, RFP, β‐galactosidase 2× HEPES‐buffered saline (HeBS; see recipe ) Anti– tag epitope antibody or anti‐substrate
. Biol. Chem. 254:10180-10183. Yang S, Tutton S, Pierce E, and Yoon K. (2001) Specific double-stranded RNA interference in undifferentiated mouse embryonic stem cells. Mol. Cell. Biol. 21(22):7807-7816. Paddison PJ, Caudy A, and Hannon GJ. (2002
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