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文献和实验Measurement of Protein Tyrosine Phosphorylation in T-Cell Subsets by Flow Cytometry
by the lack of TCR response when p59fyn activity is abrogated (3 ). Both Lck and Fyn are members of the src-family that are abundantly expressed in T lymphocytes. A third kinase belonging to the Syk-familly, ZAP-70, was shown to associate with the TCRζ
NuPAGE Gel Electrophoresis 蛋白电泳
BufferVoltage: 200V constantExpected Current: Start: 110-125 mA per gel; End: 70-80 mA per gelApprox.Run Time: 35 minutes10% Bis-Tris Gel with MOPS Running BufferVoltage: 200 V constantExpected Current: Start: 110-115 mA per gel; End: 60-70 mA per gelApprox.Run
: 110-125 mA per gel; End: 70-80 mA per gel Approx. Run Time : 35 minutes 10% Bis-Tris Gel with MOPS Running Buffer Voltage: 200 V constant Expected Current : Start: 110-115 mA per gel; End: 60-70 mA per gel
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