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文献和实验—— co-IP 检测 Hsp90 与 Cdc37 结合情况1、实验材料SKBR3 细胞,PMSF(Amresco,cat:329-98-6),cocktail(上海晶莱生物),脱脂奶粉(cat:66196131T),β-actin(上海晶莱生物)。Anti-Hsp90(santa,cat:sc-69703)、Anti-Cdc37(santa,cat:sc-13129)、Protein A+G agrose(Beyotime),细胞刮,4 度离心机(eppendorf,centrifuge
NuPAGE Gel Electrophoresis 蛋白电泳
samples add .5 ml of NuPAGE Anti-oxidant.8)Load samples onto gel and pour running buffer into the outer chamber until it covers about half of the cassette.9)Run gels according to the protocol which is applicable:10% Bis-Tris Gel with MES Running
any residual buffer from the wells. 7) Proceed to fill the inner chamber with running buffer so that the buffer level covers the level of the wells. If running reduced samples add .5 mL of NuPAGE Anti-oxidant. 8) Load samples onto gel and pour running
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