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文献和实验Construction of Mouse Adenovirus Type 1 Mutants
Construction of mouse adenovirus type 1 (MAV-1) mutants is facilitating studies of adenoviral pathogenesis in the natural host. We have isolated the first site-directed viral mutants of MAV-1, and we are comparing effects of wild-type (wt
Construction and Characterization of Adenovirus Vectors
an extinction coefficient of 1.1 x 1012 for wild-type Ad (Maizel et al. 1968 ), calculate the number of particles/milliliter: (OD260 )(dilution factor)(1.1 x 1012 ). A typical Ad vector preparation should have a ratio of particle to pfu of ~10
Growth and Purification of Enteric Adenovirus Type 40
The enteric adenoviruses of subgroup F (Ad40 and Ad41) pose some special problems of cultivation, as they cannot be readily passaged in many of the cell types used to propagate the more commonly used subgroup C serotypes (Ad2 and Ad
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