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文献和实验Preparation of Endotoxin from Pathogenic Gram-Negative Bacteria
Endotoxins have been recognized for decades as important structural components of the outer cell wall/cell membrane complex of Gram-negative microorganisms. These chemically heterogeneous macromolecular structures were recognized very early
, while gram-negative bacteria lose the purple dye after washing with 95% ethanol. The positive or negative reaction is a measure of the presence or absence of specific polysaccharide components of their cell walls. Safranin is used as a pink counterstain
Recombinant Protein Production in Antarctic Gram-Negative Bacteria
elements were isolated, cloned, and fully sequenced and their molecular characterisation was carried out; however, we focused our attention on the small multicopy plasmid, pMtBL, from the Gram-negative P. haloplanktis TAC125 strain. This episome turned
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