- ￥4884
- 赛默飞（Thermo Fisher）
- MA180485
- 2025年07月14日
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100
- 规格：
MONOCLONAL ANTIBODY- 250 UG
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文献和实验Primary Neuron Viability Assay
per well in 24 well plates which were precoated with poly-L-lysine (Becton-Dickinson Labware, Bedford, MA) and incubated in the presence or absence of test agents for 2 days at 37C in a 5% CO2 incubator. Immunostaining with an anti-neurofilament polyclonal
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
make an Antibody against a Peptides
to create an immunogen. One-step chemical reactions are easy with Pierce Sulfo-SMCC Pre-Activated Carrier Proteins (Figure 1). If your hapten does not have a sulfhydryl group, you can use EDC chemistry (Figure 2) to couple amines (lysine) or carboxylic acids
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