- ￥5842
- 赛默飞（Thermo Fisher）
- MA17323
- 2025年07月13日
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MOUSE MONOCLONAL ANTIBODY-
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文献和实验make an Antibody against a Peptides
to create an immunogen. One-step chemical reactions are easy with Pierce Sulfo-SMCC Pre-Activated Carrier Proteins (Figure 1). If your hapten does not have a sulfhydryl group, you can use EDC chemistry (Figure 2) to couple amines (lysine) or carboxylic acids
in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
, bleeding procedure, obtaining serum, precipitation of IgG. ・ GST-fusion protein injection into rabbits (IVAN J. DELGADO ORLIC) ・ How Do I make an Antibody against a Peptides (Pierce) ・ A fast and inexpensive method
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