- ￥5110
- 赛默飞（Thermo Fisher）
- MA17290
- 2025年07月11日
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文献和实验resuspension buffer and 1 volume thrombin cleavage buffer. Seal the bottom of the column. Add 1 column volume thrombin cleavage buffer plus thrombin. 10 ug per mg fusion protein is a good starting point. Incubate 30-60 minutes at room temp rocking
GST Fusion Protein Preparation
plus thrombin.10μg per mg fusion protein is a good starting point.Incubate 30-60 minutes at room temp rocking on the Nutator.If the protein is subject to degradation,try a 60 minute cleavage at 4℃.Drain BioRad columns.Soluble cleaved fusion protein
Design of Fluorescent Fusion Protein Probes
of at least three domains fused together: the donor protein, the sensory domain, and the acceptor protein. To more efficiently subclone fusion proteins containing multiple domains, a cassette-based system can be used. Generating a cassette library of commonly used domains
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