- ￥4316
- 赛默飞（Thermo Fisher）
- MA135492
- 2025年07月15日
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文献和实验the sample on a 100℃ block for 5 min.3. Apply the sample in about 10 μl volume to an appropriate percentage (typically 4-20% gradient) SDS-PAGE mini gel, along with visible protein marker (5 ul) and appropriate positive and negative controls. Run gel using
for a 1 hour transfer is: a. 0.8 mA / cm 2 gel. 8. After 1 hour, disassemble apparatus and western blot the membrane. For discontinuous semidry transfer, see the LKB 2117 Manual for appropriate reagents and assembly
until the dye is half through the gel on the above settings, and then decreasing the power to 5 mA overnight. In the latter case, the 31kD molecular weight marker should be at the bottom of the gel in the morning. Disassemble the apparatus, and discard
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