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文献和实验%B0%D7%D6%CA+%B4%BF%BB%AF FPLC系统在蛋白质纯化中的一些使用技巧 http://www.dxy.cn/bbs/post/view?bid=65&id=1077173&sty=3&keywords=%B5%B0%B0%D7%D6%CA+%B4%BF%BB%AF 关于His-tag融合蛋白质的纯化问题 http://www.dxy.cn/bbs/actions/archive/post/273883_0.html 蛋白质的疏水层析纯化步骤 http://www
domesticus x Mus spretus] F1 DNA). In the analysis of the antibody bound (B) and unbound (U) fractions (right panel), the bands representing the maternal and paternal alleles are indicated.MATERIALS Reagents100-bp DNA step ladder (for duplex PCR
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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