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文献和实验Mini-Antibody Affinity Chromatography of Lysozyme
Multiple noncovalent forces do play a role in the binding between a protein antigen and an antibody directed against that protein. An antigen is bound by the antigen binding site of an antibody that consists of three hypervariable segments
Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
Antibody Screening of Expression Libraries
Over the last decade major advances in cloning technology have markedly improved the ability to construct clone libraries expressing foreign proteins and to screen them to identify clones encoding any desired gene product. A number of types
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