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100
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MONOCLONAL ANTIBODY- 100 UG
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文献和实验----------------------- Screening for Protein-Protein Interactions. Protein G was from Pierce (Rockford, IL) and BODIPY-FL-Goat-anti-Mouse IgG was from Molecular Probes (Eugene, OR). IKBalpha and p50 were kindly provided by T. Maniatis (Harvard University, Cambridge, MA
Diego, CA); GSK-3α (Upstate, LakePlacid, NY); and b-actin (Sigma, St. Louis, MO). 7. Secondary antibodies: anti-mouse and anti-rabbit IgG conjugatedto horseradish peroxidase (Santa Cruz Biotechnology,Santa Cruz, CA). 8. Enhanced chemiluminescent
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
Staining mouse tissue with anti-mouse Ig (H&L chain) secondary reagents inevitabily causes staining of interstitial immunoglobulins, B cells, plasma cells and macrophages with Ig bound to Fc
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