- ￥5294
- 赛默飞（Thermo Fisher）
- MA1165
- 2025年07月13日
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文献和实验In Vivo and In Vitro Production of Monoclonal Antibodies: Bioreactors vs Immune Ascites
Until recently, the primary method available for the production of large quantities of monoclonal antibodies (MAbs) has been growing hybridomas in syngeneic or nude mice. This has presented a number of problems
: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
content. Prepare cell extracts in appropriate non-reducing or reducing sample buffer as indicated on the product insert. In some cases reducing agents can destroy the epitope that is recognized by a monoclonal detection antibody. Mix cell suspension
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