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文献和实验Site-Specific Cleavage of Fusion Proteins
Where an affinity tag has served its purpose, it may become desirable to remove it from the protein of interest. This chapter describes the removal of such fusion partners from the intended protein product by cleavage with site-specific
Application of TEV Protease in Protein Production
on the specific protein of interest and the desired use of the final end product (antibody production, crystallography studies etc.). This chapter reviews some common methods for the production of recombinant fusion proteins and specifically describes a versatrle
RNA-Protein Crosslink Mapping Using TEV Protease
. The position of the radioactive label crosslinked to the protein can then be determined by fragmentation of the protein using a battery of sequence-specific proteolytic enzymes or chemical reagents. However, there are typically many cleavage sites
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