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文献和实验: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT. 2. Add 1 or 2 μl of non-diluted monoclonal antibody (4B4 is better than 10H8 for HSF1) or 1/10 diluted polyclonal antibody to 10 μg of whole cell extract
Assessing Matrix Metalloproteinase Expression and Activity in Hepatocellular Carcinomas
substrate analysis. This approach has demonstrated that an increase in the expression of MMP2 (6 ,7 ), MT1-MMP (7 ), TIMP1, and TIMP2 (8 –10 ) is associated with liver fibrosis. Similarly, in hepatocellular carcinomas, a high expression of MMP2, MMP
问:有人提到一种酶缓冲液提供酶反应条件,酶蛋白即催化底物明胶,不知道是怎么回事情。我要测定活性和分泌。我知道是要使用聚丙烯酰胺凝胶电泳的,请教具体的实验方法很所需要的试剂。谢谢! 答:Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72-kD. Type IV collagenase
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