- ￥4296
- 赛默飞（Thermo Fisher）
- MA112597
- 2025年06月25日
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文献和实验Isolation and Differentiation of Amnion-Derived Stem Cells (ADSCs)
onto fibronectin-coated dishes at a density of 3,000 cell /cm2 and grown to 70% confluency in serum-containing media (SCM). Cultures are subsequently transferred to hepatocyte induction media (HIM) consisting of 20 ng/ml hepatocyte growth factor (HGF), 10 ng
Development of techniques for primary culture of C. elegans embryonic neurons
such as laminin, fibronectin, or collagen (Banker and Goslin, 1991) . Because nearly all neurons are reported to show some adhesion and axonal outgrowth on polylysine, initial experiments were done with glass coated with 0.01-1 mg/ml polylysine. C
实验采用10只2-3周龄SD大鼠，雌雄均可，体重40~60 g。 1.2 试剂 纤连蛋白（fibronectin），Ⅳ型胶原，鼠尾胶，葡聚糖（dextran，分子量100~200kDa），DNA酶Ⅰ（DNaseⅠ）购自Sigma公司；D-Hanks液，PBS，10×PBS按配方自制；Ⅱ型胶原酶购自Worthington公司；明胶，牛血清白蛋白（BSA），HEPES购自Amresco公司；Percoll购自Amersham Biosciences；DMEM（高糖）购自Gibco BRL公司；肝素
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