- ￥5811
- 赛默飞（Thermo Fisher）
- MA110697
- 2025年07月07日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 规格：
500 ug
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验In Vitro Reconstitution of Escherichia coli O86 O Antigen Repeating Unit
of the O-repeating unit is the first committed step in LPS biosynthesis. We sequenced and characterized the O-antigen biosynthetic gene cluster of Escherichia coli serotype O86. Four glycosyltransferases encoded by the genes within the cluster were cloned
1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃ 2.Grow 2-3H to A660 ~0.3-0.4 3.Chill cells 10'/ice Spin down 10',5K,4℃ 4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10',5K,4℃ 5.Wash 1X 40 ml 10% glycerol--> transfer to S34 tubes & spin
One-step Transformation of E. coli
TRANSFORMATION (ONE-STEP PEG METHOD) 1.Dilute 1:100 a fresh O/N culture of bacteria into prewarmed LB broth and incubate cells with shaking (225 rpm)to an OD600 of 0.3-0.4. 2.Add an equal volume of ice-cold 2X TSS and mix gently.[TSS is LB broth
技术资料暂无技术资料 索取技术资料
