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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
microscope. Utilizing the incorporation of fluorescein-labeled nucleotides, the use of fluorescence microscopy is also possible. B) TUNEL in situ procedure 2. PROTOCOLS A.2.1 Materials Bouin's fixative (A1), alcohols
(ECL) reagents from Pierce(Rockford, IL). 2.4. Stripping Blots 1. Stripping buffer: 7 M Guanidine HCl in water. Store at RT. 2. Wash buffer: TBS-T. 2.5. Transfection of Cells with Silencing shGSK-3a/b Vectors 1. GSK-3a-and GSK-3b-specific
免疫荧光操作(共聚焦显微镜)-Immunofluorescence Protocol for Confocal Microscopy
sections). 2. Fix 20 min 4% paraformaldehyde in 0.1M phosphate buffer pH 7.4, 0.03M sucrose on ice. ** 3. wash 2x PBS/1%BSA. From now on everything can be at room temp. or on ice. 4. Permeabilize 5 min RT in 0.2% saponin, PBS, 0.03M sucrose, 1% BSA
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