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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
microscope. Utilizing the incorporation of fluorescein-labeled nucleotides, the use of fluorescence microscopy is also possible. B) TUNEL in situ procedure 2. PROTOCOLS A.2.1 Materials Bouin's fixative (A1), alcohols
(ECL) reagents from Pierce(Rockford, IL). 2.4. Stripping Blots 1. Stripping buffer: 7 M Guanidine HCl in water. Store at RT. 2. Wash buffer: TBS-T. 2.5. Transfection of Cells with Silencing shGSK-3a/b Vectors 1. GSK-3a-and GSK-3b-specific
100.03 100.04 Dynabeads Protein G 1ml Dynabeads Protein G can bind 640 Mouse IgG. 1 ml 5 ml 1430 5720 112.01 112.02 Dynabeads M-280 Sheep anti-Mouse IgG, 6.6x108
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