- ￥3580
- 赛默飞（Thermo Fisher）
- M048T03R03-A
- 2025年07月13日
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文献和实验(ECL) reagents from Pierce(Rockford, IL). 2.4. Stripping Blots 1. Stripping buffer: 7 M Guanidine HCl in water. Store at RT. 2. Wash buffer: TBS-T. 2.5. Transfection of Cells with Silencing shGSK-3a/b Vectors 1. GSK-3a-and GSK-3b-specific
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res . 52: 1945. 3. Gorczyca, W.,Tuziak,T., Kram, A., Melamed, M.R., Darzynkiewicz, Z. 1994. Detection of apoptosis-associated DNA strand
Immunofluorescence / Confocal Microscopy Protocol
min 4% paraformaldehyde in 0.1M phosphate buffer pH 7.4, 0.03M sucrose on ice. ** 3. Wash 2x PBS/1%BSA. From now on everything can be at room temp, or on ice. 4. Permeabilize 5 min RT in 0.2% saponin, PBS, 0.03M sucrose, 1% BSA.
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