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- 文献和实验
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100
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100 µg
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文献和实验(ECL) reagents from Pierce(Rockford, IL). 2.4. Stripping Blots 1. Stripping buffer: 7 M Guanidine HCl in water. Store at RT. 2. Wash buffer: TBS-T. 2.5. Transfection of Cells with Silencing shGSK-3a/b Vectors 1. GSK-3a-and GSK-3b-specific
免疫荧光操作(共聚焦显微镜)-Immunofluorescence Protocol for Confocal Microscopy
Protocol: Immunofluorescence / confocal microscopy B or T cells in suspension, adherent cells on chambered coverglass or chamberslides, cryostat sections of unfixed, OCT embedded tissue: 1. wash cells 1x cold RPMI (no wash for cryostat
Immunofluorescence / Confocal Microscopy Protocol
实验步骤 B or T cells in suspension, adherent cells on chambered coverglass or chamberslides, cryostat sections of unfixed, OCT embedded tissue: 1. Wash cells 1x cold RPMI (no wash for cryostat sections). 2. Fix 20
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