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- 库存:
100
- 规格:
100 µg
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文献和实验). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
solution: 1M Tris pH 8, 1.5 M NaCl 20X SSPE: 175.3 g NaCl, 27.6 g NaH2PO4.H20, 7.4 g EDTA: dissolve in ~800 ml H20, adjust to pH 7.4 w/ ~6.5 ml 10 N NaOH. Adjust volume to 1 liter. V. Probing the blot; a few hours to make the probe and prehyb
direct current and shouldn't be used with the field inverter; use the Biorad or Hoeffer DC power supplies). We use the MJ Research "Programmable Power Inverter" model PPI-200 with the following parameters: A= 0.1 B= 0.01 C= 0.3 D= 0.03 E= 11
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