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- 库存:
100
- 规格:
100 µg
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文献和实验). 3. Tris-buffered saline (TBS) with Tween (TBS-T): Prepare10× stock with 1.37 M NaCl, 27 mM KCl, 250 mM Tris-HCl, pH 7.4, and 1% (v/v) Tween-20. 4. Blocking buffer: 4% (w/v) bovine serum albumin (BSA) and0.1% (w/v) sodium azide in TBS. 5. Primary
. Then take the blot out, turn the power off with the power button, and bake the blot. Of course, baking isn''t actually necessary after crosslinking, but you can do it if you want. Solutions: Denaturing solution: 0.5 M NaOH, 1.5 M NaCl Neutralizing
direct current and shouldn't be used with the field inverter; use the Biorad or Hoeffer DC power supplies). We use the MJ Research "Programmable Power Inverter" model PPI-200 with the following parameters: A= 0.1 B= 0.01 C= 0.3 D= 0.03 E= 11
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