- ￥4717
- 赛默飞（Thermo Fisher）
- M011B
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 规格：
250 µg
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文献和实验mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend
T-Cell Activation Using mAb to CD3
% final) 1ml 2-mercaptoethanol (Gibco BRL Cat. No. 21985-023) 10ml L-Glutamine (Hyclone Cat. No. SH30034.01) Antibiotic cocktail (optional) MTT Buffer: MTT (Sigma Cat. No. M5655) 5mg/ml stock
, cryopreserving, and thawing human PBMCs; and finally, detecting vaccinia-specific IL-10 and IFNγ secreting lymphocytes, as well as CD8+ IFNγ T cells following in vitro stimulation of PBMCs with vaccinia virus. The methods presented below, although optimized
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