- ￥1528
- 赛默飞（Thermo Fisher）
- L34992
- 2025年07月15日
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文献和实验Vybrant® DyeCycle Green and Orange Stains
Red or SYTOX® AADvanced™ dead cell stains or LIVE/DEAD Fixable Dead cell stains such as Green, Red, Far Red, or Near-IR kits Eliminate or correct for cell aggregates during data analysis using gating or modeling software Vybrant ®
FACS Analysis Using Peripheral Blood Cells
. (Alternatively, Becton-Dickinson sells a product called "FACS lysis buffer" that is used after the staining protocol to lyse RBCs and fix the cells.) Cells should be washed 2-3x with FACS buffer (PBS supplemented with either 1% BSA or 5% FBS and containing 0.05
for each antibody used. Incubate for 30 minutes on ice. Wash cells 2-3x with FACS buffer and suspend in 200-300 microliters FACS buffer for analysis. For live/dead discrimination, add 10 microliters propidium iodide (PI) solution (stock=10
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