- 询价
- 赛默飞（Thermo Fisher）
- HAB2105
- 2025年07月01日
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文献和实验Cyanine Dye Purification Protocol
.0 . Add 80 mL acetonitrile (HPLC grade) Adjust the final volume to 800 mL with ddH2 O. LSB, 1000 mL To 25 mL HSB , add 100 mL HPLC grade acetonitrile Raise final volume to 1L with ddH2 O
Co-Expression of human DNA primase p49-p58 subunits
as possible,inoculate 100 ml 2xYT with AMP and CAP and 10 ml of LB culture.Grow O/N at 37℃.I used a 1:10,000 dilution to avoid having an oversaturated culture after ~15hr O/N incubation. 4.Inoculate 1 L 2xYT with 100 ml O/N culture + fresh antibiotics.Use
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Reduced glutathione, 20 mM prepared in 50 mM Tris-HCl (pH 8.0) Isopropyl-β-D-thio-galactoside (IPTG) LB broth, containing appropriate antibiotic selection Lysis buffer: PBS + 1% Triton X-100, supplemented
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