- ￥5298
- 赛默飞（Thermo Fisher）
- H00729991-M16
- 2025年07月11日
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100
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100 UG
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文献和实验Aggregation of ES Cells and Eight Cell Stage Embryos
. Preparation of the aggregation plate. Aggregation and culture of the embryos with the ES cells is performed in microwells prepared in a plastic tissue culture plate using a darning needle. 1.In a 6cm petri dish add up to 10 drops of M16 media (ca. 3-4mm
in a transfer pipette and transfer to small drops of M16 medium in small petri dish. Cover with 200 FLUID and incubate at 37°C with 5% CO2. Disclaimer: The following set of protocols were contributed by various members of our lab (past and present
Collection of Morulae and Earlier
. If there is much debris floating around, then the oviduct has been properly flushed. The morulae are then collected using a mouth pipette and deposited into a petri dish in microdrops of M16 medium. These drops are covered with fluid 200 (Dow Corning, viscosity 50
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