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文献和实验Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy
interest. Here, we describe an immunofluorescence protocol to monitor the intracellular routing of the model-antigen Ovalbumin in bone marrow-derived dendritic cells (BM-DCs). This protocol describes a procedure to stain such cells with antibodies against different endosomal markers, such as EEA1
Phospholipid-Interacting Proteins by Solution-State NMR Spectroscopy
to study lipid and bilayer binding by peripheral membrane proteins in solution. We describe methods that have been optimized for the FYVE and phox (PX) domains of the EEA1 and Vam7p proteins, respectively, both of which specifically recognize
The Use of Quantum Dots for Immunochemistry Applications
for immunocytochemistry applications. We used three different antibodies—anti-β-tubulin monoclonal antibody for visualizing the microtubule network, the GM130 antibody for staining the Golgi complex, and the EEA1 antibody for detecting the endosomal system. We use
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